[Music] okay my my major topic my topic also thanks for the invitation to come here the cabin presentation I still remember when I was young you know you know when I just came to know you came to the University to study English because we just are very late there's one and him is all the ways can lead to wrong today I'm uh I'm in a room so what does it mean to me the dream comes true I hopefully that's the case okay since my previous speakers have given over a nice overall talk in detection about the bittern protection so I guess I can save some time to skip this up slides so basically I wanna say filtering is the major products produced by cuts bacteria and it has many functions a game at my previous has done excellent job I don't need to go through all of them so I can I can just go to the next slide so there's a one important issue in animal production is we saw the antibiotics from feet this is very urgent issue global wise so how can we select alternatives which can replace any parts in feet we have some certain parameters need to need to meet ideally it can affect your control enteric infection and also it can keep maintain the productivity profits in addition idea is not to change the current of procedures for for the production and the putrid had some beautiful functions which can meet those criteria but also pill drink has a limitation I still remember you know I did my PhD on rumen bacteria so each time when we prepare in the rumen Roman back there medium we have to put the bid rate into the medium it's time when we prepare the medium the whole floor was full of not you know terrible smell and the other labs all come to always come to my boss hey Cecil your your lap is awful the smell it's very offending so to solve a problem so we can use sodium butyric we can also use encapsulation technology then the other way is to make putrid Caracas rate because we have not a place in in a small intestine so those animals can release the bit rate from crocodile farm filtering to have the free beauty so putrid also have antimicrobial activity at the previous speaker mentioned and that this one we just do a one in vitro studies basically we use them by attached esta collect from chicken from chicken a second then we spiked non-pathogens semana or CrossFitting foreign genes into the medium and also we add in different concentration of a pewter ring and to see what happened and we found actually so in a control which had passed since back in spike in a digester but nope uterine supplement but the supplement but you can see precursor will only make inoculation the level of induct inoculation is 10 to 5 times 10 to 3 safe for you but after several hours crows in a control the tubes the level of nakatsu temperatures can reach 10 to 7 this is here but when at the different concentration of butyrate by filtering so even at the one hand 1000 ppm so we only detect like a 10 - to see you yeah program so this now antimicrobial activity is obvious this is this was done under anaerobic conditions and also previous studies done by Steve listen at university graph and think he was the one of the pioneer doing this work and he found actually when you add up you tree crackers right internal feet it can improve the cross animal cross performance by looking is feet convention so you can see in a control is 12.45 and this and this this the group with now antibiotics it's lower and when you have an addition of a point two percent upon four percent will reassess a so the feeding efficiency it's almost close to the group are raised on antibiotics and to follow up this work and we also did some animal trials first we because there are two different types of forms the petering wise I have a tribe uterine so that means the Krakatau had to sleep uterine molecules on the also modern appearing which only have one building a bit rate on control the first trial we study the effect of the tribe uterine employers protection we used the two different strains once Russ 308 once Russ 708 in the trial run rabbit run for five weeks and we take sample first one on day 21 and also at the end of the trial we look into different expect the parameters of gross performance and we found in general the all approach performed well that's no significance in with the intake also no significant difference in overall average daily gain and but the last significant difference we observe is not retraction of abdominal fat so which lead us to think about how could this happen and why and also what's the value tuna producers so these are those data's for the animal channels so you can see these are control these two open box I can show groups without the addition of any children so the response this is a fat versus no body weight so even between the two different strains you can see the difference so Ross 3/8 had had more abdominal fact and Ross seven or eight had land so when you on a week five so in addition to knockin shows difference the treatment groups with nappy during also shows difference so you can see the Ross three eight those two groups treat wizna uterine had a significant lower abdominal fat compared to the control group and the same as Ross seven or eight we also look into the fat deposition in the press muscle we also found the story is upset for Ross seven or eight in the plasma so in general the control group has higher fat deposition then Ross 308 and the same as the group treatment groups now Ross Seminole eight had higher fat deposition in impressed muscle so there's a strain different in the host response to no dietary bid rate and we also check the effect of Milan Puri on the chicken performance we found we observe at a similar phenomena but however in the tribe uterine treat the chickens within is seen effect on pres muscle but on those chickens treated with nominal petering we see this enhancement slightly enhancement of plasma so wait there's also those response as you can tell from this right we have like 0-5 500 1000 2000 3000 PB supplement of uterine and you can see in the muscle muscle press muscle wait there's some correlations even those not last significance when I talked to Steve listen hey I asked hey how much how much this how much does this mean to producers and what he told me even a significant difference in your case in our case is significant but even not significant a case like you can produce 50 gram more bran a plasma so in the protection that would mean large profits to not producers and and he's suggesting he spawned what very well taken birth so we think this would be good benefits to the producers and also we look in the cut morphology in a small intestine we looking at we line the left hand is not control birth without any treatment of knob uterine on right side is treated with a nap uterine you can see the morphology on the v/line treated with no filtering is better the integrity is better and then we follow up with the McClendon studies because obviously we seen a significant reduction of abdominal fat deposition and we won't see what's happening this is a similar animal chance but with small number of new parts but it tells weeks three then we sacrifice approach and also on day 14 we took na with some bottom cuts digesta to do microbiota analysis and also those birds taken from the 21 with we check now host the immune response host response by RS sick so what we see from those are three tissues of exam - the serum once not Georgia new ones abdominal adipose we found Karpis carboxy nice fatty acids in de cinces decreased and also we found that fatty acids in synthetase reduced and also the pole protein lipase which is in charge for the free fatty acid and transportation the level decreased the toll the total cross wrong in a serum decreased and then we we did I a sec this space we see how many reads we cut is more than ten million reads weaker suppose we show this data are reliable together we have enough reads and then soon our PA analysis and the devs firing for me by romantics resources analysis and we found the multiple genes with motor functions in Georgina and liver or affected by building the top affected the function the pathways one is the deepest metabolism second among the small molecule biochemistry the third wine its molecule transport the third one under last one to cell to cell signaling and the interaction and we also did like a DNS pathway analysis and we we identify that PPA our second amine is on a top in Joe jr. so it's also in a liver on a second position as well because the PP air it's important the pathway for lipid metabolism so we we did more analysis we are you know from pathways so which is showing this slide we have to you know for this one the solid square and also taught outside this means the regulation of gene expression and for this one in inner nine is not hotline outside is certain line that doesn't mean the genes upregulated and when you look those those genes you can see except the except that this one which is upregulated is for oxidation of fatty acid or others the old tongue regulated so basically disk this tells us the conclusion is the building can regulate the fatty acid synthesis and also reduce the storage and also reduce now transportation and secretion in encounter studies is increase in our station of fatty acid so all this put together that suggests why the abdominal fat deposition was decreased by Netaji petering and okay this is a sorry about the host response we also look into the microbiome to see what what happened and basically we took a two approaches once the 60s an aging sequence the other way we do Namit applied analysis with enema so for beta for our diversity we didn't see any difference between the control group and the patron shield group sona PD is a basal doubt the PG is the patron Krakatoa treated groups so basically for those in desk Joe showing - you know Shawn are indexed within see any difference however when we do Peter ty was the analysis we can see the treatment in fact because they basically just well separated so that means the boundary event did have effect on the micro my microbial population distribution this basically shows that the distribution at family and and the Janice level and the Left who is you know some you know idiom the right to box microbiota from Sikkim so I don't want there no needs to go to unity health the basic let's show Raphael there disappear disappear dispute distributions okay the summary from the 16s RNA genes sequence analysis we got such a conclusions here before for this class Paisley is pathogens we found no density of this group was reduced and also Janice you bacteria and these two groups particular these groups is related in a Pew Turing production and for this group is basically associated with an unhealthy seeker and why significant observations we got excited is now increased not species diversity on a pivotal back there and also tensity as well so we follow up with the qpcr analysis we measure one of the key enzymes for butyrin production and we didn't see any difference between those two groups but the four dost CrossFit improv engine conceding groups clusters one of them also it's a major pretty rated producing a bacteria we also didn't see any difference but for pivotal bacteria again we seen increase of intensity and also from the 60s on a sequence data we found the species diversity was also increased so basically the conclusion we can make for sickle microbiota the building had no effect on a bacterial diversity but it can alter the microbiota accommodation but these changes are good for gut health we also follow up a little serum vertebrate analysis this table shows the pain of my meta prize which showed difference should an enhancement the rest were not among this metabolite was added PCA analysis a principal component analysis and we found those two groups metabolites can also group together can separate the well so that means the treatment the effect so the summary for this microbiome analysis basically we can say that dietary treatment of the petering can increase v or bacterial population and also species diversity and we also see the increase of the serum chili meta right and according to one river paper published in science 2012 bifida bacteria and factly of bacteria can moderate lipid metabolism and cause hemostasis so therefore we thought there's a link between the microbiota and the production of the build rate which can enhance the chicken performance as we see in the Tea Party in a reduction of the abdominal fat deposition so that conclusion we can make so the pig whole message would be that a tributary can promote the cross performance I think right now we can only see in young animals and second benefit would be a reduction of the abdominal fat deposition third one would be increased summer breast muscle weight of course this can be sooner actionable gene recreation of a lipid metabolism in addition it can improve heart health by in by increased neck cuts enhanced cut development and also moderate microbiota for good gut health okay this the average is many found by our department and also Canadian poultry industry and I believe on my poster fellow Alex in his one of the major contributor tuna host response tuna bittering and he worked for your company before in Shanghai it's small work okay and also my collaborators still distance is one of the top and logicians protein magician and he helped us a lot okay that's all my talk today thank you [Music]