[Music] first question since Bill's rate induce pyy which stimulate appetite does that mean animal will have a lower feed intake yes it reduces appetite so I think if you look at so you can look at the context of what I want us to do is not to look at bitrate in isolation okay and we still need to I believe we need to conduct a lot of experiments as we begin to supplement chickens and pigs with bit rate to see does he have an effect on appetite I haven't seen that okay but some of the studies where they have measured the feather be Tyrion appetite the level is very high these are pharmacological level okay so maybe we are not going to get to that point but you see may be point zero five point one or point three percent bit rate in a in the animal feed but there's that chance there when you use pharmacological level I mean I'm talking about very high level so I don't think I don't know yes is there any way that you can pry this to increase the feed intake well I generally believe that you know betrayal would help promote feed intake but they I believe the mechanism might be by suppressing inflammation because that is very clear you know by promoting the health of the gut for instance by reducing inflammation in the brain normalizing brain function you know we know that brain controls appetite so I think there's probably more direct link between betrayed and promotion of feed intake when you look at its effect in the gut in helping to suppress inflammation but also in helping to promote the development of the god the maturity of the god these are things that we help stimulate feed intake enhance or increase our feed efficiency okay two other questions similar to what we just ask the final question is is the effect on limited storage you mentioned that's the opposite to what that the girls had to print anything yes again we have to look at it context okay in in vitro system when you use a lot of bitrate again we use that in vitro system to study mechanism okay so when you use a lot of butyrate what we really going to do is gonna serve as a source as a precursor for making fatty acids okay and when you make those failures you have to store them okay so the concentration might be that resort might be related to increased concentration but when you when butyrate is used in mice even though it was a high level it actually led to reduce fat deposition okay so we have to separate the two contexts in vitro versus in vivo okay I asked some very simple question so in general talking about its origin what normal physiological level open up you tree in the brain oh it's very very low I would say in the nano molar range yes in the nano molar range is very low okay okay [Music]