[Music] good morning everybody I'm representing Canadian University but I may say it's really good to be home I hope you all enjoy being in Poland thinking dying as much as I do today however I will be talking about their local casino late rapeseed meal as a viable source of protein for poultry this is presentation prepared by myself and by the dr. Zelinsky us as it's been said before we came from Canada from University of Manitoba canola is the internationally recognized term for varieties containing less than 2% of Aroostook acid in the oil portion and 30 micromo per one gram of the of the glucosinolates in the meal and it has been in in Europe the term double-zero rapeseed is used to be identified the canola quality milk however the term canola it's been recognized and described allow glucose annoy traipse it worldwide regardless of the origin and it has been it's been seen as a one of the greatest agricultural success the traditional breeding because it provides with the one of the healthiest vegetable oil for the human consumption it provides the high protein meal for the livestock and also great source for the biofuels feedstock for buy of biofuels and and a global production of rapeseed as for the light last year was over 65 million of metric stone I may say that it's canola has been also the political crop because the the amount of the of the canola produce may change especially in Canada it's been currently some turmoil between Canada and China was what I remain in buyer but by regardless in Canada around 21 million metric ton has been produced in Europe approaching 20 million metric tons and crop canola crop has been affected as well but as lately but ourselves by the severe weather conditions so it's not as it was quite disappointing lately china produces around 2.2 4.2 million metric tons and and australia produced quite a quite a lot of lot as well what is the situation in europe with with with the canola in europe around 20 million metric tonne is crushed and yoli and roughly 60% of the oil goes producing europe goes to the biofuel sector and cannot canola is mainly processed by the pre-press solvent extraction process also the expell processing has been gaining the popularity are however is usually the smaller scale and and it's not that as important as the preferred solvent extraction processing roughly around 13 million of metric tonne of canola meal is produced in Europe annually yep I will present the chemical composition of the canola meal from the different sources we've been [Music] surveilling the canola meal and checking the quality of canola meal from all Canadian processing facilities in Canada for over seven years and then we also analyzed the Glock glucosinolate rapeseed meal from Poland Australia and expelled processed canola and as well the low fiber gyro seeded canola I will be not presenting the yellow seeded canola results you may find more detailed results in the in the proceedings a part of the classical crude protein amino acid fast sugars neutral detergent fiber analysis more sophisticated the complex and analytical procedures were used to to determine the total dietary fiber non-starch polysaccharides legal in polyphenols and under glucosinolates and I was just about to present the crude crude protein level in of canola meal and the rapeseed meal from Poland from from Canada in Europe it's very similar and it a ver egde 40 41.6% in the canadian meal and and 40% in a European and it also has been widely recognized that the glucosinolates lever is not an it does not cause concern for the canola meal users anymore since the glucose new level Lucas analytes level has been a steadily declining it's not only the effect of the in plant-breeding bow so effect of the of the canola meal processing since the the most heat treatment will decomposed the glucosinolates as you can see when when we we look at the exposure placed canola low casinos like Murchison elites level it's it's a bit higher because the there's a lack of the or less impact of the heat treatment involve here so canola is still lower in them in the protein than soybean meal however we may consider it as a good source of protein canola meal has relatively high amounts of phosphorus 1.12 and 1.21 roughly percent of the total phosphorus and the phosphorus in the form of non fighted and phytate although it's still high in fighted phosphorus is one of the greatest source of phosphorus for animals well compared to other other feed ingredients protein with with the well balanced amino acid composition is the most viable nutrient of of canola meal when it when compared to soybean it contain less arginine lysine entraining but it's it contains more Natalia an assistant juice it is really advisable to use canola meal and soybean meal complementary rather than exclude one of our or another the total amount of of amino acid it's not necessarily the best indicator of the canola meal quality focus should be paid rather on an amino acid digestibility since digestibility of an amino acid may be highly affected by the processing conditions when the oil is extracted because excessive heat treatment during the pre pre solvent extraction of canola may reduce the digestibility of amino acids and in the first of all contributes significantly to the variation on the notary in the nutritive value of canola meal from the pre-press solvent extraction stream is more or less similar in in any kind of crashing facility however it's all depends of how they're certain steps are designed I mean what are the conditions and then and the pre-press solvent extraction contains few stages like make up stages when the heat is applied which may affect the quality so the stages are like a seed drying and then cooking and flaking solvent extraction and also the solvent rising in the disadvantaging toaster this is the step when the hexane is removed from the from the meal and it's very critical because it has been heated for prolonged time and it may affect the quality of the of the new the most heat treatment may cause the formation of the Maillard reaction products which are resembling the lignans so they are contributing to to the fiber content and but first of all they they reduce the amino set availability Maillard reaction occur when in the presence of amino acids and the reducing sugars which are provided by canola means so so this this reaction is initiated out started with the most heat treatment and may affect the quality of the canola meal another factor which which may contribute to the variation in a fiber content it's a dual de screening and and dockage the variation in the inner canola meal qualities one of the most limiting factors of the of the for using of canola meal does that's why we focus our research focus quite intensively on the on the fiber content and components and checking the relation to the dough the quality screening and dockage are added at the end of the production usually and this is also important that it may be different in Europe than in Canada because in Canada majority of the canola ground is Randa Brady in in other words I may say that weeds are more control means they sell less screenings and dockage present in the in the seat so so that that may also cause the the difference in the fiber content another part of the another component of canola meal which may increase the fiber those are the garments of stocks added to the meal after the oil refining that it contains and phospholipids contribute to the fat content however this this kind of fat is poorly digestible by by animals and as a consequence of the small size of the seat and high oil content the milk contains relatively high proportion of dietary fiber and neutral detergent fiber when compared to soybean meal and they're based on the on the survey conducted in Canada the total dietary fiber it's around thirty eight point four percent and they're slightly a bit higher in the in the European poultry actually can can efficiently utilize diet which are containing hand high about high amount of canola even right right at the early stage of the development I will be talking about this in a minute the main main component of the total dietary fiber are non-starch polysaccharides the other components that are legal in the polyphenols and and the glycoproteins they may they may be a great source of the variation because the Maillard reactions products are hidden somewhere here they resemble a lignin and also this neutral detergent insoluble crude protein it's an actually the protein which is British at which is attached to the to the nutrient d/f and it's not available so the mod and the ICP it's indicating the overheating of canola of canola meal so the formation of the of the Maillard reaction products will in will lead to the increase in dietary fiber and NDF and lignans and in our earlier research in the laboratory setting the samples of the fatted Canales it has been treated with the most heat treatment at the 95 100 105 110 and 126 degrees of Celsius and the samples analyzed for the NDF and and the ICP and and in the results application of the temperature higher than 105 degrees significantly increase the level of NDF and of course this kindly anti undigestible fraction of the dietary fiber and so this is definitely in there i indicated of the overheating of the canola meal in our recent studies on the chemical composition of canola meal from a canadian crashing grunts the differences between crashing plants has been noted and and especially between the the NDF and total dietary fibers and neutral detergent in soluble crude protein and lignin polyphenols they were showing the highest vibration that means that it reflects the differences in the in the processing setups and conditions the the regression between the and EF and total fiber is presented here so it's a highly correlations Harika correlated and the same dietary fiber and and the ICP is highly correlated indicating that this is the main source of the variation of the meal lice and being one of them most susceptible for the heat treatment amino acid it's negatively correlated with the NDF levels as you can see the less and EF the more lysine in the in the MU and as well license correlated with the total dietary fiber although the equations has been developed so the content of the lysine and could be predicted using simple measurements such as NDF or ADF and i explain more in the proceedings you may find the references it also has been observed that the amount of the glucosinolates is highly related to the dietary fiber in our survey we noticed that plant canola meal sample from plant where the high glucosinolates level has been observed contains lowest level of dietary fiber and the same story here lowest level of the of the glucosinolates was reflected in one of the highest because you know a total fiber content it reflects that the samples could be overheated because the canola meal glucosinolates are mostly composed of the indoor glucosinolates and are very susceptible for the heat treatment and easily decomposed by the moist treatment just briefly the a lot of work done successful work on the development of the yellow seeded canola meal and however research showed that their breeding follow fiber canola resulted most likely in the quantitative characteristics as evidence by increased oil protein sucrose content rather than any significant improvement in the nutrient utilization and and due to the decrease fiber content but you may find more in the Proceedings about this just briefly because I said that expelling it's becoming her more popular as well the main difference between expelled and pressed canola and conventional canola or rapeseed meal is the oil content and this is also the main limitation because because the the oil content would be the biggest source of the variability and nutrition is don't really like the the product which is not really uniform and the variability it's coming from the different processes because sometimes canola seed could be expelled twice sometimes just once so the oil content may range from 82 to 20% and the cells may be not completely ruptures and and their high glucosinolate is present in the mill because the mitosis is still active although there may be some potential effects of the heat due to friction during them expelling I will briefly say something about the big chunk of work done in in our lab that the enzyme research we use the enzyme to improve the value of canola I'm not going to spend more much time on the details but I wanted to present one graph we treated canola with with the single carbohydrates or carbohydrates in the in the combination the first bar represent the and it total announced our polysaccharide content in the canola which was not treated with an enzyme and as you can see a different enzyme combination we're bringing down making making lower lower NSPS content in the canola meal what we can conclude from this graph whatever was released during this process we call it and NSPS hydrolysis products and they may contribute to and to them may have a positive impact on a gut held because those are there they've got the properties of the prebiotics and his peas are complex polysaccharides but enzyme a hydrolyzed them to the short chain polysaccharides always go saccharides and simple sugars so not only the enzyme hydrolysis products may be utilized as a prebiotic bowel so contribute early to be to the to the metabolism energy it as you can see the AME for brawlers and turkeys has been improved when enzymes were added and we evaluate the nutritive value of canola meal first of all we analyzed the AME value and then standard in your amino acid digestibility and around set of the growth performance study this is the number which we came up with after the several studies so I ran a over 1900 kilocalories per kg of the dry matter of a me4 broiler chickens and almost 2100 kilocalorie for turkeys amino acid digestibility for broilers has been determined and total digestibility is around 80 point eight percent I mean a 82 percent so then digestible amino acid content could be could be calculated and adjust for the diet formulation a little bit lower total amino acid digestibility 78% for turkeys and following that we had the good resource and they were under quite a few studies with proper chilled turkeys a broiler chicken tortoise and there one study wood layers we explored a high inclusion level of the of the canola meal for birds so few studies I summarized the the studies here one study of wood brothers 35 days of age canola meal level of the 15 percent results are very very satisfactory we fed the broilers in the pre started phase adding 15 percent of the canola as well good results and there would be another study we did well in a little bit more and two studies with turkeys results were also satisfactory we're under study with the sort of like a titration study we start with with the zero so no no canola and ending up at the 30 percent of canola was also good I summarized we summarized the the overall performance so so in comparison to control bird fed from 15 to 30% in the last growing phase they performed very well and I have to remind you that we formulate the diets based on the on the digestible amino acid content and the available energy this is the same show data showing the in a graph format lengthen study was run two periods of the twelve weeks each we went with a canola up to 20% we measured the glucosinolates content just to prove the concept so even in the highest canola meal level at 20% we had around 1.5 micro mole per gram of the glucosinolates and it has been proposed that the 1.5 this is still the the level which will not cause the adverse effect on the health and production of flairs results has been proved as some significant differences because the second treatment was a little bit off but it really has nothing to do with the with the highest one the production was satisfactory and and there now major differences of course equality has been determined the lighter bar represent the face to that the darker bar a president face one and as you can see no major differences in a specific gravity higher units actual elasticity and actual thickness has been observed okay I'll go just one minute wanted to summarize canola meal or low glucosinolate rapeseed meal it's available source of the protein for poultry it could be used effectively at the 15 20 % of full of inclusion in the broiler torque in length and diets however it does have to be have to be formulated carefully using the digestible amino acid and available energy contents and due to the low glucosinolate content high inclusion level of canola meal would not adverse adversely affect the animal health and growth canola seed processing conditions are the main contribute to the variation of the of the meal quality and the excessive heat during previous segment extraction would result in reduced digestibility of salmon particle realizing and neutral that under fiber and acid detergent fiber measurements could be indicators of the canola meal quality could be used for the in the for the calculation of the quality in into the regression equations and most of the canola fiber is simply a dilute ins would have minimal effect on the new Trinity design Oh done thank you so much [Applause]