without Oh No everybody Thank You chairman thank you for your introduction to begin with I'd like to thank for the invitation from Professor Philip mercy and the organizing committee today I assure you a share with you some knowledge about intestinal intestinal loop sandhya physical barrier in poultry and the modification solutions where discuss four parts first study about what's the barrier of competition and the function and the secondary is the development of teaching machines over the barrier and the surgery mechanism which impair the teaching machines and barrier functions mastery I try to share with you some solutions to improve the barrier function the episode of parallel function is one of the four major intestinal barriers a civil Oh Mike repelled by a barrier chemical barrier physical barrier and the immunological Perea the physical barrier is the major barrier difference the body from the outside that if you send a physical barrier is permeable barrier which permitting the absorption of nutrients electrolytes underwater while maintaining effective against defenses the body against intradermal or toxins entities and enterica flora flora so if the barrier impaired or disrupted some toxins or pathogens will penetrate a course episodio into the blood and the resulting in by the IPCC's and systematical inflammation some proteins contribute to the mission of Terra junctions can be categorized as types of proteins they are set of skeleton structure proteins cytoplasmic scaffold proteins and internal integral membrane proteins most of the attentions have been paid on the cytoplasmic cyka cyka for proteins and integral membrane protein because of the determinantal low for the barrier functions of period the episode of Berea one of the big family is Claudius the Claudius can be classified as two types wine is very forming Claudius which decreases the parasite develop permeability big the cell tightly shanked it so strengthened the barrier function the second one is a pore forming Claudius which can permit increase cellular permeability and and some researchers reported that that's very important for for driving diarrhea and the clearance of positing political barriers the development of the tablet Junction proteins takes will take a long time even though some proteins can be detected in the embryo during the Imperial development but however some reports some researchers reported that some of the proteins cannot be reached paths which plateau until 10 days or even two weeks in my lab we we also studied what has to the development of physical barrier regarding with sometimes junctions in the probably chickens the papers show that our occluding 301 and keep hearing among splashing cannot Plateau at you the fourth day 14 however Claudine 3:03 and can can cannot reach plateau on 2020 p21 first 21 peace the the patter of do to develop develop abilities slowly and can reach the highest level do the day of 28 of age so so it it is obviously human here it takes some to two or three days two or three weeks for the joint third Junction proteins to to develop and it also suggested that maybe some special twitching due to pecans in the to be considered for in terms of the terry junction protein development regarding with the mechanism which account for the para junction proteins development or the disruptions met many of literature's can be can be found during the last two taxes now it is it is very clear that the three product the key factor is pro-inflammatory cytokines which induced disruption of junctions where some cell-cell signaling pathways as indicated in this decorate the grammatical sketch some calluses also Xuan was showing two are related with was involved in the signaling pathways such as rocky and jianchi and the human medicine medicine callous so how the inflammation response happened was the cause for the information as we know there are two major type of molecules responsible for the reading from the Machine the first study the first one is a pumps the called as passage associated molecule patterns such as appears and and endo toxins from Pacifica back the bacteria and the second one is a tempest that is damage associated more clear patterns those molecules released but such as the host cells undergo in necrosis they can act as intuitive as danger signals to induce inflammation in terms of the the course for for pumps and the tempest product pumps and the tempest production some environmental and dietary factors can induce that the inflammation inflammation inflammation responses from the environment site as you lower poor hygiene and the heat heat heat stress can induce can induce passage e pap pumps produced and reduce Tammy Oaks Maurice strong positive stress in the host cells in terms of the paired pathogens we everybody lo'ed that a besides mycotoxin we specified a logical proteins such as conditioning can pretty suppose the hostler cell to very strong walk straight oxidative stress and the which will result in the inflammatory responses for red for instance if chicks ingested with with mycotoxin they will be very very high level for inflammatory cytokines produced compared to the control in the meantime some some joint some jeong-seon proteins such as keith haring occluding and accordions can be decreased when when the feast contaminated with the toxin chronic ingestion of low dose of mycotoxins ordered in tasking so you can from this slice the morphological damage worry is significant in the groups ingested a with mycotoxin and the legends go very high when the mycotoxin ingested and then these treatments will be pretty suppose animals to be infected easily by enteric pathogens so what solutions can be used to improve the intestinal ibis idea physical barrier in my my view is is that we we we can we can do that through two ways the first is to clear the Technol the toxins and allergic antigen freeze in the death in the dead career the toxic under logical antigens in the dead through a quality control for for example we can now use the microcosm contempt the field staff fittest herbs such as a coin and we can use proper technology present technologies to destroy allergic antigens such as beta conditioning in the soybean products certainly is to fortify the debt with nutrients and the food additives here i like to show you some data collected in my lab or from literature's to show how the nutrients can improve nutrients and the food items activists can prove the sorry up area firstly is the nutrients can be used to inhibit inhabit inflammatory resort responses they are those reported nutrients include cheese minerals such as zinc selenium and the vitamins for instance the vitamin A when me P and the e etcetera under some function though I'm a disease for example like chilling and short chain fatty-acids zinc is very very important she's melosa for animals because of his very wide range of biological rows of zinc zinc in in my experiment we challenged it with cross with improper engines in my lab we show we observed that zinc deficiency were impaired we impair the the third Junction protein expression and when the zinc announced increased the third Junction protein such as Croydon one where we increase the area in the dose response manner from this the practical the consequence is that as the zinc supplementation level increased the lower level of in focusing in this in the in the plasma can be detected and both had and the less mortality of the chickens can be found in in the trial as you lower zinc can be it can be deposited in the egg even now the not quite efficiently but but with catechol zinc because of the more power available and zinc can be can be easily deposited in index so so some carryover in fact can be expected and and some epigenetic nutritional or my tablet program programming effects can I expected expected in the chickens and they improve the progeny performance in you my expire in my experiment we did observe some beneficial effects of supplemental zinc for the rail operators such as showing anticipate in this slice the third Junction crowding one expressing increased with the with the zinc supplementation into the breeders breeders that and the zinc the low catechizing treatment appeared little bit more efficient about the for the permeability of the barrier is improved improved when we when we inoculate some molecular probes in this requirement we use the horseradish peroxidase as as big body cooler substances can be absorbed absorber the patella can metabolic but metabolized by the chicken so it can be used as a probe to test the permit from the ability of the in hospital physical barrier in this experiment the data clearly show that with zinc supplementation the the hardest the horseradish peroxidase levels in this in the serum decreased with zinc supplementation that means that the permeability increased by the decision to increase window surveillance of the repeaters IO experiment some epigenetic a nutritional rose also this code is closed because of histone acetylation in the promoter region of protein a twenty Jing was observed 20 protein is a zinc finger protein which can which can which have some anti-inflammatory effect so so some some inflammatory cytokines can be reduced by zinc supplementation and identity Junction proteins expressing enhanced by the zinc supplement supplementation in the in a dad for Breda breeders the second ways is is to shape cut a micro pear water through reducing Pacific bacteria and at the anatomy G associate the molecule patterns of disease just as talked about talked talked talked before and secondly can increase by the fusion microbes to produce intestinal Tawfik prog-rock ranked prog-rock again the rival peppered head and the consequently improved it is radio telling gene protein expression for study some food additives and direct in active directory with cotton lycra water such as probiotics prebiotics searching for the LCS and fara genetics the mechanism of probiotics and metabolize is computed he was excluding all positive occur bacteria some many beneficial microbes have been reported such as pasilla settlers like the Policy Forum and so on and some metabolites produced by the probiotics such as organic amid organic acids and the bacteria singh and the so on can be can be used for for the protection of intestinal intestinal epithelial physical barrier under law under common commercial production Scott mark priority spouses can be can be caused by by poor sanitation and the high temperature as shown in this table when the chickens suffer from his hit stress the the lactobacillus and above and my Phaedo bacterium in the digester of the chicken decreased however the cross with iam and occur and coliforms increased when the chickens supplemented with probiotics the changes can be reversed so so the physical barriers also improved and the permeability decreased as a showing in the trans audio electrical resistance and because o2 serum Nikola group conversations issue in this in this table expression some Junction proteins also observed in some experiments with when the probiotics are used for the chickens such as pasilla settlers and East bloody the the metabolites of properties also tested in my lab and the we observed that the the probiotics conditioned the media can be used to improve the permeability of the cell and Kakadu malala's as shown by the electrical resistance and the level of molecular probe Flora's process in substances under the anti-inflammatory effect of the metabolites our probiotics is also verified in this experiment as showing this slice when the interleukin 1 was it was over was idea into the mid in the media for for for the kakapo line how could who 7 on the permeability increased however when the in included into the culture and permitted permeability decrease quite a lot essential oil as well oh they see some essential oil can clear some passages and it can be used exactly widely used in commercial productions to improve the cutter hell's in my lab we tested the valuation of micro macro rooms after essential supplementary into the data for broiler chickens no matter at a phenomenal family level or was the pc solaris never big differences occur after the essential way of supplemented at the species level as you see you can see from this slide a lot of peripheral microbes increase such as lactobacillus Chris Peters and and a genus lactobacillus Chris Peters is hydrogen peroxide producing microbiome species which can protect the host cell from from in fact infectious pathogens and and lactobacillus killers is hetero of competitive my group which you can produce some pie was so fact and fact endure which not only exhibit anti adhesive and but also have some bacterial activity against Staphylococcus aureus so so what they are very good for for the curry house in terms of the meta-genome changes we we did over serve some differences happened we can't always the violence affect gene abundance with the essential way of supplementation some very important or harmful virulence factors gene abundance decreased had a lot for instance obvious appears is a kind of endotoxin and and otherwise pH is the bios or the hydrolysis they they also they they can regulate the cell membrane and the nebula paralysis so very very very bad and and the CEO PE is atp-dependent protease that plays some role in fossil transmission no modification protein turnover and chaperons which is detrimental so from from the metal genome beta it is clearly showing some essential oils very good I was all detected some immune related gene abundance the patella show that in the control group group relative abundance of in be impossible decrease compared to the essential oil increase that to increase in compared to the essential oil that means supplemental you see you can already relieve the immune responses caused by cause freedom of regions that means some inflammatory responses can be expected in this experiment actually in another experiment we tested some some less inflammatory cytokine produced with the teaching of essential oil and and improved Advaita the expression of the junction proteins such as occlusions and including one and the the fellow the consequence is the permeability changed with the essential oil supplementation the mobility decrease decrease and the less lower plasma in the toxin can be detected in the in the plasma of the chickens this secondly some food additives can in can have some indirectly affects our microbiota for instance some glucans vitamins and the feed enzymes as for glucans we detected big big influences of beta glucan some reputation and endogenous and him back repaired secretions secretions and the unites another experiment we test we reserved some beneficial effects of Perak routines of repetition to improve improve the barrier function very functions as shown in this table the crowding one chronium four-and-oh Clawdeen's russians war i debated even the chicks challenges with cross challenges with salmonella so the translocation of the Salmonella well was inhibited after the death supplemented of Aspira croakin the effect of it I mean with honey also reported in few in some of some articles it is it is sure it was shown that vitamin A deficiency can result in lower difference-in-differences secretions and I debated I debated some positive ik such as equalized ecoli ecoli microbes and decrease however decrease the population of some part official microbes such as lactobacillus enzymes enzymes well oh I can for instance non-starch polysaccharides can destroy pitch effect of polysaccharide and the release model trains for for animals to use protease can reduce nutrients supply for pathogens such as sodium for engine because the like have some lack of genes for biosynthesis output of amino acids for protein synthesis for protein so they have to secrete toxins and some enzymes to damage in the mucus or the cell with a sale of the hostile sale to uptake nutrients for their own crews so protease is a very potential effective added him to him to control some positive ik bacteria in the in the in the in the guard to improve the kind of house in the Dallas can produce prebiotics such where he goes I cried for parish very few microbes just as talked this morning by a professor touka tell you in my lab we test the beneficio of holidays supplementation and the callee house these people show show you the tablet junction proteins expression can be influenced by the Saliva's when the the chickens challenges with oranges the some some of the terrace junctions will decrease sticker victory such as oak loading in the indium however when the when the chicks fed with the data with holidays the oak rooting expression wrist was restored to LUMO so the consequence is the plasma in the Tollison level in the plasma decreased by the enzyme supplementation and the fellow practical consequences is nice modality in in in the group father with journalists even the chickens challenges with course with improv regions so under this condition it is can be sad journalist is a very efficient alternative would for antibiotics in terms of the complexity of the non-starch polysaccharide days we are continue to try to find some some more efficient accomplishing of enzymes to produce more probiotics for the beneficial microbes in the gut in my experiment we combine the delays with regular for our loss atrocities and viral oil histories in the midsole in vitro experiment we tested the production the hydrolysis of the recipe after the analysis as as you can see from this table Zelo bios's jello trioses and the jello potatoes the production secret significantly increased by the EMS application especially the three computation of enzymes produced more probiotics you miss who experimented with with the culture with bacillus subtilis we we found that the beneficial effect of the the three compilation of the enzymes is much better than the effect of the of a Lada Oracle saccharide the force as you know our force is widely used and while verify it is a very efficient Oracle saccharide so from from this experiment we can see that somebody some proper compilation of enzymes can be used abuse to to improve the cut apart microbiota and the effect may be better than some what he goes a crate the feeding trial showed that actually macro power Beata changed or improved by the sublimation of enzymes as a Xuan negative bacillus in the in the sickle in the sig in a single contents much significantly improved by the sub imitating of computations and enzyme supplementation while however the e.coli contents decreased quite significantly so the new episode of permeability also improved improved by the completion of enzymes and the legions goal can be decreased the third solution to improved callicles is try to find some nutritional regulators so far so far several several of these kind of substances was reported to improve the kind of house through through inhibiting repeating inflammatory cytokine productions but with regard the mechanisms it it is a closed that the function was through modulating the PS Anki is Ricky and I Katie pathways for residents resigning resigning glutamine is sorry Reutimann is widely used for for pigs for police and this environment the substation of glutamine improved 30 jangling of the shadows at a level of 30 Junction proteins proteins all claudinês and sey to expressing was clearly improved by the cotton mill supplementation in vitro c6 shokyo is a product extracted from ginger powder and this the paper also reported that the Showgirl can be prevent an inflammatory cytokine production through P is really equity pass away and which finally inhibit they have Kappa B signaling and reduced process for inflammatory cytokine productions EGF as epidermal growth factor now is not approved Federative used in commercial production however is however is widely used for cosmetics number number number was nasty nasty reported that EGF can prevent capita bacteria bacteria vector induced disruptions and that expression reduction of the junction in function proteins such as Croton for even the aging of the capital factor given the change in mass much however the child you feel invisible number was evenly lowered so the sublimation of EGF so the translocations decreased with EGF supplementation many nutrients and the food additives have been shown to improve the barrier function integrity and the functions because of time limit time limit I don't want to wish she would just share some examples with you in terms of different mechanisms so I like to summarize here today firstly the inflammation response induce the disruption and test function of the intestinal epithelium physical barrier and secondary in terms of the barrier funking higher allowance of and inflammatory nutrients should be provided then for optimal growth and feed conversion ratio such as zinc with amines and some with immunizes and nastily some functional food additives which can you reduce the inflammatory response due to pumps and the damps can be used to improve the physical barrier and the enhance the cutter integrity thank you thank you [Music]