[Music] thank you for the nice introduction I appreciate the company for putting this workshop together and also funnel imitation to give me the opportunity to give a presentation and a discuss with my college we all know if we have a happy we have a health cut as a human we all feel happy but for the animals if they have a health the cards so that means they will have a good performance and golf so what's the definition of the health card so in general it should be kept in houma status while maximize is a function so tell around for expect to describe have the guts the first would be in an optimal digestion and absorption functions and the second would be effective parry function the third one is effective but low cost the immune system the last one would be small and the diverse and stable microbiota so you my talk I would touch the last three points so from this slide under the red line space East the functions of a beauty what he can do the first one at the first speaker you know described is the major energy source for colon and recites in addition to its function at nutrient it also acts as messengers which Recker eight variety are marcelo functions with include and high in from entry if and the pro protection protection of an epithelium barrier in addition it records the tissue cut tissue proliferation and the development so how I have East the major mechanisms in the molecular mechanisms were putrid in fact in this slide it's mainly include the pill drinking inhibit histone deacetylase that means it can increase transcription levels secondly it can interact with cells surface receptors at Philippe's just mentioned so therefore two inference the cell signaling and third one it would wreck rates proliferation and a patata artosis factors in addition it enhanced intentional act line phosphatase and I will touch upon you might follow insight and it's also induce cut inducible heat shock proteins which also regulate variety several activities it can regulate bacteria virus signals finally it in moderate gut microbiota composition and in turn to affect bacteria metabolites so it has it has a lot of several functions which benefits the gut health so what's the source our beauty it can be produced by : fermentation by gut bacteria and variables just point out I showed you some slides and also we can also supply the beauty in the feet to make the works and this sites based is show you know how you know no Collin fermentation when God has an a dietary fiber so at the fermentation results it will change the back tail composition and reduce the pH value in a gut it can also in hence no short-chain fatty acid production the joyful production many includes now estate butyrate and the prom in the acid Prasanna gas and this is our early a publication in early 2002 thousand as I joined AFC that's my first paper and actually it's also first publication using 16s RNA sequence to explore or to investigate the micro bill committee community structure and constructions in boilers but it's mute its mucosa associated bacterial population so as you can tell so the pewter introducing bacteria the this one it's pretty government and also faculty bacterial process in the eye it's also a pillar in producing bacteria at that time because this almost back to almost twenty years ago that 16s database is it's quite small and the tools we have we don't have the aluminum negative sequence cost so that time week what we can only do you can generate a gene library so you can tell it's a total clone number is 126 clones but it's it's just tip on that I iceberg even though the number is small but it is indicate in the change that children producing bacteria is very important so nowadays now 16s RNA database become very big so and the used to be the site and those groups they always identified uncultured and culturable bacteria now become clear so majority I'm gonna be reproducing back appearance the cross medium crossed 14 and also for and including other Clostridium bacteria cause freedom costs to do bacterias and the one particular you know it's indicate in a slight it's a peculiar bacterial process any I which is the one of major produce beauty the producing bacteria it's also mucosa colonizer so I'm going to let's look at know what's the what is not effective a parry functions so the diagram shows in a fun very structure so it's composed of M cells and and recites kalapa cells and also some immune cells and the T Junction third Junction to indicate palabras plays a critical role in the in the gut integrity so when the junctions damaged the cuts become a deacon then it's going to be a problem and your khazneh information effect no animal cause performance so when we have a deacon cut so in general the information will increased the immune response will increase and the nutrition efficiency will decrease as a result this will affect will affect adversely animal performance so the question is how can we prevent the cat streaking it's the possible to repair the custom image the next two slides I'm going to show a given example water putrid can repair the gut damage so this work was done by Jack Hoel in China their groups they work in piglets the basically the idea they artificially induced cuts damage by infusion as the asset and then meanwhile the trick was not being with your type on the Builder age and as you see if an obit read can repair on the guts damage so as you can see from this slide the this is control group this is a acidic challenge in the piglets disability treated the group's so for real I have and the Crippen adapts and also you look non-capital cells and also enjoy epithelial lymphocyte cells and also lymphocytes then density so you can see in the aesthetic challenge in the epic reads the those parameters either up or down significantly so when you treat the way over the peaks what we do within our building so all the parameters almost back to the control groups so that means now the application administration of the building can reverse the cuts damage to elevate the cattle injury and this is an others are an alum are these are also appears on the results are often the immune approach the page with the check no proteins that key proteins for a pitocin and also for T Junction formation the top one is now Katherine Katherine page 3 which is the key enzyme for apotheosis the bottom lines crossing one which is a key factor for T Junction formation so as you can he'll acidic treatment basically increase in the production cafes three and the town regulated the crusting one which the treatment of a building so both proteins work back to close to not control piglets which you know have no treatment so and also the check now eg F receptor level in as Aesthetica traded no piglets none a receptor level was found regulated significantly but in the control groups and children treated groups there's no different and no significant the difference there for tech inclusion is the beauty the treatment that can inhibit the hypothesis promote tight Junction formation and active a EGF receptor signaling therefore to elevate the intestinal injury so let's look at an effective and a low-cost immune system so ideally we hope the situation there's no adaptive but only email response only innate immune response and also know last cut information and and at at such the energies used for immune response can be used for animal groups I think in the first law first speaker is clear indicators in his speech so what for the cut information because we know no son the subtle cast information is always there and the cut information most result from Appetit and damage and the bacteria and the toxin and their toxins for example LPS are one of the common factors to cosmic gas formation so communities now information and I think if we can increase the gut integrity that would be one option and also if we can increase the innate immune response this would include removal of the information inducing components such as LPS and also reduce the production you pour in from enter e cytokines and increase of anti-roman tree molecules additionally increase nominal products antimicrobial peptide would be another choice and finally and also the other arm a major point would be in Krishna intestinal lining phosphatase that we can simplify to I a pea IEP is the marker for cuts development and maturity in addition it is also not entire nation enzymes so it had several functions but those specifically associated information including detoxification of bacterial endotoxins such as LPS and also recreation intense intestinal bacterial translocation the translocation will cause sepsis and the LPS because local or even some non systematic information as I mentioned at the beginning the way I aap works and for those Punk you know in the lumen also panda a membrane the many teeth on the phosphor rate LPS and the detoxify it's not activity and therefore to proc the opening to toll-like receptor 4 and result no alas LPS in and induced in the cell signaling therefore reduced information and for those located inside of the cells it can block nf-kb translocation to the nucleus and therefore down regulate the pro-environment or e response such as in the looking aid and TNF alpha and this is one example I think it's a day you serve individual studies the author use human concern cells and they simply they test different length of the short term various including cities his receiver up to c6c for Speed Rail this is the immuno abroad so as you can tell on the top now I pee in the sea for a butyrate enhancing the production of na i AP so that means it probably will increase the activity I'm not a P 240 certification of LPS which help to reduce now cut information and the lastly I'm going to touch upon the know microbiota as we say we ideally we wish to have small diverse and stable microbiota so we always struggle with how can we balance for sufficient the microbiota which allow for sufficient email and cut tissue development the element would be for the colonization resistance to enteric infections by pathogens and also we also hope it will have a good nutrient nutrient efficiency including digestion and the fermentation overloaded cottolengo burner will compete with animals for nutrients that can be used for anymore course so the questions do we have any weapon towards the computer it helping so so next few slides I will cite no work from that muscle groups and he is a he just gave a talk so they haven't done some extended jobs so in the first night the basically they look on the effect of a beauty on Samara Burton Anders and the shedding and as you can tell from this slides from the control group which has no supplement on the peerage now Salmonella Samantha burden it's quite high than those groups of birds which has been treated with speed rate so they follow up to see you know what's the reason for it they tried they in Western the mechanisms so they did in mutual studies with Keiko to reproduce k2 cells and they look ah Salmonella pathogenicity I ice Island the one and this island one basically contains most genes which are responsible for cut evasion they found here a these particular genes are significantly unregulated even though most other genes were also unregulated and the follow up with mutation they make the mutant kill a and to see if they can seen a similar result in the in vivo studies by doing this you know as you can see from the right side or panels the Y type actually they now some relish shading is pretty higher than the mutant mutant which had been no indicate in the town is much lower similar shedding my type so this basically approved that here here a is now signal and the putrid can suppress suppress is signal therefore to achieve the result to reduce the Seminole a burden and infection and that they also you know the Filipina point around say okay if we provide we have some bacteria but putrid producing back there and so what's the story and as you can see from this slide you can see on day 40 so I'm not going to go to an honor data I just specific point out day 40 you look at those they divided into male and the female but they also have the older birds you looked as a feat conversion ratio and the beauty in the producing bacteria treated the group had much better fitting efficiency compared to the control group which received no Beauty producing bacteria they also looked into no microbiota and on day 25 at 8:26 and at the top 10 oh here I didn't say okay top 10 oh here so they found the two major difference once I believe it's the one is the back thyroid and we are why it's not bitter in producer bacteria so you can defend like the butyric supplement and the chicken supplement with no butyrina the Spector had significand Bacteroidetes population yeah and also on day 40 they also found the difference and the nectar boisterous group and also the birds receive Peter in the producing bacteria horse Ohama high abundance of lactobacillus in the cuts in addition they also look in a supplement of butyrate producing bacteria on challenge in the birds is any disease they haven't done for consider considered given a chance with an approach were challenging with cost written for engines and in and for those can choke group which had no has no supplement of nobility producing bacteria had significant hi any additions and also the instance of any disease compared to the group which received which receive which had which had received Nebuta impedance back there so all and you know the whole story I think it's pretty good you know so basically the indicate in the B train came specifically in suppress some enteric pathogens and also because they they can also by suppressing some specific in genes the expression and the next day feels light so I want to talk our own work it's also this is not this is axel beard rate we have seen one phenomena when we do know chicken chance by when the press get in tune week three and we found the purse is receiving a bill rate had significant reduction of abdominal fat deposition so this was you can tell from a show in the left panel we test the two different chickens chicken strains when trust 308 once 708 the can open panel or open bars our control group birds which receive no butyrate and those colored one whines no supplement with no 500 PV any other one supplement with 2000 ppm our petering so regardless the supplementation or not now 708 has much mass that reduced the abdominal fat deposition compared to rest 308 and also supplementation on the be drinkin further reduced factorization interprets we also look into if supplementation breaking hands the press muscle at previous reports described there there's positive effect in our hand we also confirm this beauty supplementation ability can in hands press muscle production as indicated by the right panel so what's not mag didn't behind this so we carried out their further studies first that we examine the host response we look those key enzymes in lipid metabolism so this led esterline for no major key factors once no carbon carboxylase and fatty acid synthesis and also the protein lipase and the total serum cross room and we found all these inland key factors or downregulated what's decreased by the supplementary panel treatment of nobility and we also within the is sick analysis and we found out the one of the major second on pathways as a PP PR which respond from the lipids meta prison well what some of England's were some one key point of attack what unregulated has indicate by those squares that we start lights outside that's done regulated with a solid outside is now up regulated so in the conclusion from this put together with the be in effect on now on a deep pit rate on the lipid metabolism would be tongue regulate lipid synthesis and the lipids of storage and its transportation and the secure interestingly now of oxidation of lipids and the fatty acid also increased so this Patek is a nice story and fit in what are observed the first question we asked if a microbiota can't be - this phenomena we use with didna sickness sickness and a sequence to look on the community structure and accommodation we also analyzed serum tap right with no animal techniques and on the left hand you can see in the beta diversity of the microbiota so you can clearly cluster separate each other so that means now there is a treatment effect basically treatment of children had had the effect on the microbiota and compositions we also identify 30 I think more than 30 ki keep bacteria a key composition of micro biota and as indicated on final top on the heat heat map panels and and also we follow up with a real-time PCR analysis we identified that among that those children producing bacteria like a class 14 class for within see any changes in their opponents in the population however we identified pause abundance and the species diversity over butyrin significant increased by the treatment of Madrid and the Lochner this in the result shows na and results of Syrian analysis and those those items in red are my top right pipe is bacterial metabolites so you can see all we exempt the top right a break up regulated so and also we use in a principal component analysis and also shows the cluster the treatment effect is separate from those on treatment so as a conclusion for these studies and also we look at a lot of literature's the one science paper dimensional acts a uterine and fine caliber bacteria can moderate the deepest metabolism and glucose homeostasis this actually fit in our observations and therefore we propose and this Nabil bill pivotal bacteria can contribute to the decrease of abdominal fat deposition in the chickens so that's our the story we got from our studies so the take-home message would be the bid rate can benefit gutted digestive and episode functions it can enhance cuts in integrity it can reduce the information and the end-rig infections and also improve nakata microbiota finally which you wanna go to promote animal cause performance but one thing i also want to point out at the previous speaker said everything is dynamic and the parents so everything has its limits so whenever you make a conclusions then you need look what under what conditions yeah that need to keep kept in mind that's all my talk today thank you for attention [Applause]